Mass spectrometers for doubly-labeled water
DLW mass spectrometry
Physical activity assessment objective
Doubly labelled water
16th November 2021
Soren Brage, University of Cambridge
DLW is a method for measuring carbon dioxide production during free-living using stable isotopes (deuterium or Hydrogen-2 and Oxygen-18). It requires mass spectrometry instruments for measuring the enrichment of these isotopes in the collected biological samples (either saliva, urine, or blood). Oxygen-18 enrichment: AP2003, Analytical Precision Ltd, Northwich, Cheshire, UK; Deuterium enrichment: Isoprime, GV Instruments, Wythenshaw, Manchester, UK or Sercon ABCA-Hydra 20–22, Sercon Ltd, Crewe, UK.